The Fusarium disease, caused by Fusarium oxysporum f. sp. lycopersici has been observed in different areas of Iran in recent years. Current biocontrol studies have confirmed the effectiveness of the Trichoderma species against many fungal phytopathogens. In this study, biocontrol effects of Trichoderma isolates alone and in combination and non-pathogen Fusarium were evaluated against F. oxysporum pathogen. In this investigation, these isolates had shown good control of Fusarium oxysporum f. sp. lycopersici in vitro condition and Tomato roots were treated with T. harzianum individually and in combination with T. asperellum and nonpathogen Fusarium planted in artificially infested soil with pathogen Fusarium oxysporum f. sp. lycopersici, all treatments showed significant differences with control, treatments for T. harzianum (T-7) showed more protective effect. Combination of T. harzianum (T-7) + T. asperellum (T- 3) isolate gave the best control (62. 3) at greenhouse condition.